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Bacterial DNA Sequences In The Prostate Of Men Without Chronic Prostatitis

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Susan Keay
Chen-Ou Zhang
Brenda R. Baldwin
Richard B. Alexander Baltimore, MD
Studies using nested polymerase chain reaction (PCR) analysis of transperineal prostate biopsies from patients with chronic non-bacterial prostatitis indicated the presence if DNA from a variety of prokaryotic microorganisms in 77% of specimens (Krieger, et al, J. Clin. Microbiol. 34:
3120, 1996). Because this study did not include a control group we wished to determine whether microbial DNA could also be found in transperineal prostate biopsies obtained from men undergoing prostatic brachytherapy for localized prostatic cancer who did not have a history of prostatitis.
Nine patients with clinically localized adencarcinoma of the prostate gave informed consent to participate in the study. None had any antecedent history of prostatitis or chronic pelvic pain. All had a negative catheterized urine culture and none had been on antibiotic therapy. Transperineal biopsies of both lobes of the prostate were obtained under ultrasound guidance using the double needle technique of Krieger et al. The biopsies were placed in sterile water, DNA was extracted and 2-round amplification performed using nested primers from a highly conserved region of the bacterial 16s rRNA gene. Amplified DNA was purified ans sequenced using the Sequenase PCR Product Sequencing kit, and the sequences obtained were compared to bacterial rRNA gene sequences recorded in GenBank.
Eleven of 18 biopsy specimens from 8 of 9 patients were positive by PCR for bacterial DNA encoding bacterial 16s rRNA. Sequence data indicated a predominant organism in 8 of the 11 specimens, with >95% homology to DNA from several different genera of bacteria including Escherichia ans Bacteroides. All nine control samples from the instruments before biopsy were negative.
The presence of bacterial 16s rRNA genes in prostate tissue is not specific for chronic prostatitis and occurred in most of our patients with localized prostatic cancer. Whether the presence of such bacteria in the prostate is related to the development of prostatic diseases such as prostatits or prostatic cancer will require carefully controlled trials including appropriate control groups examined identically.
Supported by a grant from the U.S. Department of Veterans Affairs.
Source: 1998 AUA Meeting
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