1999 AUA Selected Abstracts


1999 Selected Abstracts from American Urological Association annual meeting
SEARCH FOR AUTOREACTIVE ANTIBODIES IN THE SERA OF PATIENTS WITH BENIGN PROSTATIC HYPERPLASIA
Georg Steiner, Stephan Madersbacher, Gero Kramer, Anne Kofler, Michael Marberger; Vienna, Austria (Presentation to be made by Dr. Madersbacher)
INTRODUCTION AND OBJECTIVES:
The development of benign prostatic hyperplasia (BPH) is associated with the expression of new or overexpression of existing antigens. Potentially, these antigens are recognised by the immune system and/or released into the circulation. The aim of our study was therefore to assess the presence of auto-reactive antibodies in the sera of patients with BPH.
METHODS:
A total of 31 patients with BPH and 8 controls (prepubertal boys) entered this study. Serum samples at study entry were analysed by immunohistochemistry using fetal-, fibromyoademoatous-, myomatous- and adenomatous BPH-tissues by anti-human-IgG, -IgA or -IgM. Western blotting experiments were performed by blotting a mixture (n=8) of representative BPH-tissue extracts. These blots were incubated with the respective BPH and control sera and, subsequently, by isotype specific anti-human IgG, IgM and IgA.
RESULTS:
Semiquantitative comparison of the immunohistological analysis revealed a heterogeneous staining pattern with all three Ig subtype specific antibodies when using BPH-sera. Alterations of the anti-IgG, -IgA, and -IgM immunoreactivity as compared to control sera were found on all three major prostatic cell types and in all cellular compartments. These differences (BPH versus controls) were most prominent and frequent when using anti-human IgA. Western blotting experiments revealed that BPH-tissue extracts contained significant amounts of human -IgG and -IgA as well as small amounts of -IgM. The most impressive differences between control- and BPH-sera were found when using anti-IgA. With respect to these differences we identified anti-IgA reactive bands above 170kD, which were absent in all control patients. A differential expressed band at 120kD was additionally detected.
CONCLUSIONS:
The significant reactivity of BPH-patient sera with a variety of BPH-tissue antigens prove the presence of anti-prostatic antibodies in the circulation of these individuals. These data are of interest for a novel classification of BPH based on serological markers.
Source: 1999 AUA Meeting
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SEARCH FOR AUTOREACTIVE ANTIBODIES IN THE SERA OF PATIENTS WITH BENIGN PROSTATIC HYPERPLASIA