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| J Clin Microbiol 1999 Jun;37(6):1863-1870
|
| Prevalence of Corynebacterial 16S rRNA Sequences in
Patients with Bacterial and "Nonbacterial" Prostatitis.
|
| Tanner MA, Shoskes D, Shahed A, Pace NR
|
Departments of Plant and Microbial Biology and of Molecular and Cell
Biology
University of
California
Berkeley, California 94720-3102. |
| The etiology of chronic prostatitis syndromes in men is controversial,
particularly when positive cultures for established uropathogens are
lacking. Although identification of bacteria in prostatic fluid has
relied on cultivation and microscopy, most microorganisms in the
environment, including some human pathogens, are resistant to
cultivation. |
| We report here on an rRNA-based molecular phylogenetic
approach to the identification of bacteria in prostate fluid
from prostatitis patients. Positive bacterial signals were seen for 65%
of patients with chronic prostatitis overall. Seven of 11 patients with
bacterial signals but none of 6 patients without bacterial signals were
cured with antibiotic-based therapy. |
| Results indicate the occurrence in
the prostate fluid of a wide spectrum of bacterial species representing
several genera. Most rRNA genes were closely related to those of species
belonging to the genera Corynebacterium,
Staphylococcus, Peptostreptococcus, Streptococcus, and Escherichia.
Unexpectedly, a wide diversity of Corynebacterium species was found in
high proportion compared to the proportions of other bacterial species
found. A subset of these 16S rRNA sequences represent those
of undescribed species on the basis of their positions in phylogenetic
trees. These uncharacterized organisms were not detected in control
samples, suggesting that the organisms have a role in the
disease or are the consequence of the disease. |
| These studies show that
microorganisms associated with prostatitis generally occur as complex
microbial communities that differ between patients. The results also
indicate that microbial communities distinct from those
associated with prostatitis may occur at low levels in normal prostatic
fluid. |
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