|
| AUTHORS: |
| Bjorling DE; Jerde TJ; Zine MJ; Busser BW; Saban MR; Saban R |
| AUTHOR AFFILIATION: |
| Department of Surgical Sciences , School of Veterinary Medicine,
University of Wisconsin, Madison 53706, USA. |
| SOURCE: |
| J Urol 1999 Jul;162(1):231-6 |
| ABSTRACT: |
| PURPOSE:
We hypothesized that experimental cystitis induced by substance
P(SP) or E. coli lipopolysaccharide (LPS) would be less severe in mice
rendered mast cell deficient by genetic manipulation. |
| MATERIALS AND
METHODS:
Two strains of mast-cell deficient mice (WBB6F1- kitW/kitW-v or
kitW/kitW-v and WCB6F1-Sl/Sld or Sl/Sld) and their congenic, normal
(+/+) counterparts were used. Cystitis was induced in female mice by
intravenous injection of SP (0.1 ml.;10(-6) M) or E. coli LPS (0.1 ml.;
2 mg./ml.), and inflammation was assessed by Evans blue dye
extravasation. In a separate group of kitW/kitW-v and congenic normal
mice, cystitis was induced by intravesical infusion of SP (0.05 ml.;
10(-5)M) or E. coli LPS (0.05 ml.; 100 microg./ml.) and compared with
intravesical pyrogen-free saline (0.05 ml.; 0.9%). Severity of cystitis
was determined by histological evaluation of the bladder wall 24 hours
after intravesical infusions. |
| RESULTS:
Intravenous SP or LPS stimulated
increased plasma extravasation in congenic normal mice but not in mast
cell-deficient mice. Intravesical SP or LPS resulted in increased edema,
leukocytic infiltration, and hemorrhage within the bladder wall in
congenic normal mice, but the only histological evidence of inflammation
in the bladders of kitW/kitW-v mice was increased hemorrhage in response
to LPS. |
| CONCLUSIONS:
This study indicates that mast cells modulate the
inflammatory response of the bladder to SP and LPS in mice. Although
clinical trials of the use of antihistamines to treat or prevent
cystitis have not been successful, these results suggest that therapies
directed toward preventing mast cell activation may yet prove effective
in treating cystitis. |
| Best regards,
Jordan Dimitrakov, MD |
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